anti mouse igm ap ap polyclonal southernbiotech  (SouthernBiotech)

Journal: EMBO Molecular Medicine

Article Title: Protein-based tools for the detection and characterisation of Oropouche virus infection

doi: 10.1038/s44321-025-00291-7

Figure Lengend Snippet: ( A ) Immunoblots of lysates from HeLa cells mock-infected or infected with OROV BeAn19991 (MOI 5). Membranes were probed with HRP-conjugated nanobody-Fc fusions or with a polyclonal antibody against OROV plus anti-mouse secondary antibody, as indicated. Membranes were subsequently probed for GAPDH as a loading control. ( B ) Immunocytochemistry of HeLa cells infected with OROV BeAn19991 (MOI 0.5). Cells were probed with AlexaFluor (AF)568 conjugated OROV nanobodies against N (NpE3) or Gc Spike (SpE8), with a polyclonal antibody against OROV and with an antibody against TGN46, plus appropriate secondary antibodies. Nuclei are stained with DAPI (blue). Scale bar = 10 µm. ( C ) Nanobody-Fc fusions that recognise Gc spike neutralise OROV infection in vitro. Plaque reduction neutralisation assay using Gc spike Nb-Fc fusions, performed using OROV BeAn19991 (top) or AM0088 (bottom). Data for three independent experiments plus SD and ND 50 values are shown. .

Article Snippet: HRP-conjugated polyclonal goat anti-mouse IgM , Merck , Cat# AP128P.

Techniques: Western Blot, Infection, Control, Immunocytochemistry, Staining, In Vitro

Journal: EMBO Molecular Medicine

Article Title: Protein-based tools for the detection and characterisation of Oropouche virus infection

doi: 10.1038/s44321-025-00291-7

Figure Lengend Snippet: HeLa cells were infected with OROV AM0088 (MOI 0.5). Cells were probed with AlexaFluor (AF)568 conjugated OROV nanobodies against N (NpE3) or Gc Spike (SpE8), with a polyclonal antibody against OROV and with an antibody against TGN46, plus appropriate secondary antibodies. Nuclei are stained with DAPI (blue). Scale bar = 10 µm.

Article Snippet: HRP-conjugated polyclonal goat anti-mouse IgM , Merck , Cat# AP128P.

Techniques: Infection, Staining

Journal: iScience

Article Title: Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1

doi: 10.1016/j.isci.2025.112151

Figure Lengend Snippet:

Article Snippet: Goat anti-mouse IgM-HRP (polyclonal) (ELISA) , ThermoFisher Scientific , Cat# 62-6820; RRID: AB_2533954.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Functional Assay, Western Blot, Recombinant, Protease Inhibitor, Diagnostic Assay, Staining, Software